Difference between revisions of "Part:BBa K1321009"
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This part is BBa_K1151001 in Frieburg (RFC[25]) format to allow for easy use in fusion proteins. It is a histidine-rich metal binding protein which binds up to 5 Ni2+ ions (please see the original BBa_K1151001 biobrick page from team Università del Salento Lecce 2013 for more detailed information). For expression purposes this part is available with T7 promoter (BBa_K1321163) or LacI promoter (BBa_K1321162).  
 
This part is BBa_K1151001 in Frieburg (RFC[25]) format to allow for easy use in fusion proteins. It is a histidine-rich metal binding protein which binds up to 5 Ni2+ ions (please see the original BBa_K1151001 biobrick page from team Università del Salento Lecce 2013 for more detailed information). For expression purposes this part is available with T7 promoter (BBa_K1321163) or LacI promoter (BBa_K1321162).  
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Please note that the DNA sequence shown starts after the rfc25 prefix and ends just before the rfc25 suffix. The start codon and stop codon are including in the rfc25 prefix and suffix sequences respectively.
  
 
<br /> Additionally it is available in fusions with various cellulose binding domains as front (BBa_K1321343, BBa_K1321344, BBa_K1321345) or back inserts (BBa_K1321351, BBa_K1321368, BBa_K1321369) including versions suitable for expression using T7 (BBa_K1321352, BBa_K1321353, BBa_K1321364) or LacI (BBa_K1321362, BBa_K1321361) promoters.
 
<br /> Additionally it is available in fusions with various cellulose binding domains as front (BBa_K1321343, BBa_K1321344, BBa_K1321345) or back inserts (BBa_K1321351, BBa_K1321368, BBa_K1321369) including versions suitable for expression using T7 (BBa_K1321352, BBa_K1321353, BBa_K1321364) or LacI (BBa_K1321362, BBa_K1321361) promoters.

Revision as of 15:53, 11 October 2014

Nickel Binding Protein (NiBP) Freiburg format (RFC[25])

This part is BBa_K1151001 in Frieburg (RFC[25]) format to allow for easy use in fusion proteins. It is a histidine-rich metal binding protein which binds up to 5 Ni2+ ions (please see the original BBa_K1151001 biobrick page from team Università del Salento Lecce 2013 for more detailed information). For expression purposes this part is available with T7 promoter (BBa_K1321163) or LacI promoter (BBa_K1321162).

Please note that the DNA sequence shown starts after the rfc25 prefix and ends just before the rfc25 suffix. The start codon and stop codon are including in the rfc25 prefix and suffix sequences respectively.


Additionally it is available in fusions with various cellulose binding domains as front (BBa_K1321343, BBa_K1321344, BBa_K1321345) or back inserts (BBa_K1321351, BBa_K1321368, BBa_K1321369) including versions suitable for expression using T7 (BBa_K1321352, BBa_K1321353, BBa_K1321364) or LacI (BBa_K1321362, BBa_K1321361) promoters.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]