Difference between revisions of "Part:BBa K1321009"
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This part is BBa_K1151001 in Frieburg (RFC[25]) format to allow for easy use in fusion proteins. It is a histidine-rich metal binding protein which binds up to 5 Ni2+ ions (please see the original BBa_K1151001 biobrick page from team Università del Salento Lecce 2013 for more detailed information). For expression purposes this part is available with T7 promoter (BBa_K1321163) or LacI promoter (BBa_K1321162).  
 
This part is BBa_K1151001 in Frieburg (RFC[25]) format to allow for easy use in fusion proteins. It is a histidine-rich metal binding protein which binds up to 5 Ni2+ ions (please see the original BBa_K1151001 biobrick page from team Università del Salento Lecce 2013 for more detailed information). For expression purposes this part is available with T7 promoter (BBa_K1321163) or LacI promoter (BBa_K1321162).  
  
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<br /> Additionally it is available in fusions with various cellulose binding domains as front (BBa_K1321343, BBa_K1321344, BBa_K1321345) or back inserts (K1321351, K1321368, K1321369) including versions suitable for expression using T7 (BBa_K1321352, BBa_K1321353, BBa_K1321364) or LacI (K1321362, K1321361) promoters.
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<br /> Additionally it is available in fusions with various cellulose binding domains as front (BBa_K1321343, BBa_K1321344, BBa_K1321345) or back inserts (BBa_K1321351, BBa_K1321368, BBa_K1321369) including versions suitable for expression using T7 (BBa_K1321352, BBa_K1321353, BBa_K1321364) or LacI (BBa_K1321362, BBa_K1321361) promoters.
  
  

Revision as of 15:22, 11 October 2014

Nickel Binding Protein (NiBP) Freiburg format (RFC[25])

This part is BBa_K1151001 in Frieburg (RFC[25]) format to allow for easy use in fusion proteins. It is a histidine-rich metal binding protein which binds up to 5 Ni2+ ions (please see the original BBa_K1151001 biobrick page from team Università del Salento Lecce 2013 for more detailed information). For expression purposes this part is available with T7 promoter (BBa_K1321163) or LacI promoter (BBa_K1321162).


Additionally it is available in fusions with various cellulose binding domains as front (BBa_K1321343, BBa_K1321344, BBa_K1321345) or back inserts (BBa_K1321351, BBa_K1321368, BBa_K1321369) including versions suitable for expression using T7 (BBa_K1321352, BBa_K1321353, BBa_K1321364) or LacI (BBa_K1321362, BBa_K1321361) promoters.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]