Difference between revisions of "Part:BBa K1497024"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1497024 short</partinfo>
 
<partinfo>BBa_K1497024 short</partinfo>
  
−
d
+
<html>
−
 
+
<div align="left">
 +
<table class="MsoTableGrid"
 +
style="border: medium none ; border-collapse: collapse; text-align: left;"
 +
border="0" cellpadding="0" cellspacing="0">
 +
  <tbody>
 +
    <tr style="height: 214.9pt;">
 +
   
 +
<td style="padding: 0cm 5.4pt; vertical-align: top; width: 306.7pt; height: 214.9pt;">
 +
The GBD domain and its ligand (Part:BBa_K771106) are suitable tools for protein colocalizaion. Initially, the domain was a part of the N-WASP protein (GTPase binding domain) in Rattus rattus. The GBD domain is used as a binding unit of the so-called protein scaffold published by Dueber et al. in 2012. The scaffold (BBa_K1497033) is composed of different binding units, which enable the assembly of multiple target proteins. This BioBrick adds a BglII and BamHI restriction site in front of and behind the previously constructed domain sequence (Part:BBa_K771105). Now, different binding units of the scaffold protein can be fused together without the introduction of restriction sites.  This allows the easy construction of BioBricks of different permutations of the scaffold protein domains.
 +
</td>
 +
<td
 +
style="padding: 0cm 5.4pt; vertical-align: top; width: 136.7pt; height: 114.9pt;">
 +
      <img
 +
style="width: 450px; height: 300px;" alt=""
 +
src="https://static.igem.org/mediawiki/parts/9/9e/GBD.gif"></p>
 +
      <br>
 +
      <p class="MsoCaption" align="text-align:justify"><span lang="EN-US"><b>Figure 1</b></span></a><span lang="EN-US">
 +
.</span></p>
 +
      </td>
 +
    </tr>
 +
<tbody>
 +
</table>
 +
</div>
 +
</html>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 12:03, 11 October 2014

GBD-Domain

The GBD domain and its ligand (Part:BBa_K771106) are suitable tools for protein colocalizaion. Initially, the domain was a part of the N-WASP protein (GTPase binding domain) in Rattus rattus. The GBD domain is used as a binding unit of the so-called protein scaffold published by Dueber et al. in 2012. The scaffold (BBa_K1497033) is composed of different binding units, which enable the assembly of multiple target proteins. This BioBrick adds a BglII and BamHI restriction site in front of and behind the previously constructed domain sequence (Part:BBa_K771105). Now, different binding units of the scaffold protein can be fused together without the introduction of restriction sites. This allows the easy construction of BioBricks of different permutations of the scaffold protein domains.


Figure 1 .
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]