Difference between revisions of "Part:BBa K1497004"

 
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<partinfo>BBa_K1497004 short</partinfo>
 
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The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway. The CHS needs a high amount of Malonyl-CoA to constantly perform the reaction. So it has to be noted, that if used in <i> E. coli </i> the source of Malonyl-CoA is limited.
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      <p class="MsoCaption" align="text-align:justify"><span lang="EN-US"><b>Figure 1</b></span></a><span lang="EN-US">
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Reaction of the CHS. 4-coumaroyl-CoA reacts together with 3 Malonyl-CoA to one Naringenin Calcone.</span></p>
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The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway.    
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For using the CHI in our Naringenin Operon (K1497007) we added the strong RBS B0034.  
 
For using the CHI in our Naringenin Operon (K1497007) we added the strong RBS B0034.  
  

Revision as of 11:13, 11 October 2014

B0034-CHS - Chalcone Synthase from Gerbera with strong RBS

The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway. The CHS needs a high amount of Malonyl-CoA to constantly perform the reaction. So it has to be noted, that if used in E. coli the source of Malonyl-CoA is limited.


Figure 1 Reaction of the CHS. 4-coumaroyl-CoA reacts together with 3 Malonyl-CoA to one Naringenin Calcone.

For using the CHI in our Naringenin Operon (K1497007) we added the strong RBS B0034.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 543
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 552
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 523
    Illegal NgoMIV site found at 1099
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 472