Difference between revisions of "Part:BBa K1497001"

Revision as of 10:35, 11 October 2014

CHS - Chalcone Synthase from Gerbera

The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway. It has to be noted, that if used in E. coli the source of Malonyl-CoA is limited.

The iGEM Team TU Darmstadt 2014 used the CHI and verified the function of the CHS in their naringenin operon (K1497007).

Figure 1 Reaction of the CHS. 4-coumaroyl-CoA reacts together with 3 Malonyl-CoA to one Naringenin Calcone.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 525
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 534
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 505
Illegal NgoMIV site found at 1081
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 454

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-The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway.
+The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway. It has to be noted, that if used in <i> E. coli </i> the source of Malonyl-CoA is limited.
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