Difference between revisions of "Part:BBa K1497005"

Revision as of 10:19, 11 October 2014

B0034-CHS-B0034-CHI - Composite part of RBS-CHS and RBS-CHI

This device is able to generate Naringenin from 4-courmaroyl-CoA, if put under a promotor. This device is the second composite part for the assembly of our Naringenin Operon (K1497007).

Part 1: K1497004: B0034-CHS - Chalcone Synthase from Gerbera with strong RBS The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway.

For using the CHI in our Naringenin Operon (K1497007) we added the strong RBS B0034.

Part 2: K1497003: B0034-CHI - Chalcone Isomerase from Petunia with strong RBS The Chalcone Isomerse catalyzes the reaction from Naringenin chalcone to Naringenin. This enzyme is non-essential for flavanone pathways, because the reaction takes place independently from CHI in the cytosol. The enzyme independent reaction is catalyzed by an acidic compound. Without CHI the reaction is stereo unspecific. S-Naringenin is the active compound for the most enzymes in the biochemical pathways. To prevent gaining a racemic product and losing 50% yield, CHI is used, because only the enzyme is able to form 100 % S-Naringenin.

For using the CHI in our Naringenin Operon (K1497007) we added the strong RBS B0034.

Sequence and Features