Difference between revisions of "Part:BBa K1448000"

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<partinfo>BBa_K1448000 short</partinfo>
 
<partinfo>BBa_K1448000 short</partinfo>
  
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gamma-terpinene synthase produces gamma-terpinene from GPP,geranyl diphosphate.
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This part contains ɤ-terpinene synthase region of ''Citrus unshiu'' and was isolated by Mr.Shimada from ''Citrus unshiu''.[[http://www.sciencedirect.com/science/article/pii/S0168945203003777 Shimada et al, 2003]]
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<br><br><br>
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This part contains gamma-terpinene synthase region of ''Citrus unshiu'' and was isolated by Mr.Shimada from ''Citrus unshiu''.[[http://www.sciencedirect.com/science/article/pii/S0168945203003777 Shimada et al, 2003]]
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'''Further information:'''
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* DDBJ entry: [[http://getentry.ddbj.nig.ac.jp/getentry/na/AB110639/?format=flatfile&filetype=html&trace=true&show_suppressed=false&limit=10 AB110639]]
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* Origin of the enzyme: ''Citrus unshiu''
  
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[[File:Citrus unshiu.png|thumb|400px|right|Image of ''Citrus unshiu'']]
  
  
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[[File:terinene synthesis.png|thumb|600px|left|Fig.2 Reaction catalyzed by ɤ-terpinene synthase.]]
 
[[File:terinene synthesis.png|thumb|600px|left|Fig.2 Reaction catalyzed by ɤ-terpinene synthase.]]
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== '''Characterization''' ==
 
== '''Characterization''' ==
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=== Gel picture of our designned part ===
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=== Western-bloting analysis ===
 
=== Western-bloting analysis ===

Revision as of 04:32, 11 October 2014

gamma-terpinene synthase

This part contains ɤ-terpinene synthase region of Citrus unshiu and was isolated by Mr.Shimada from Citrus unshiu.http://www.sciencedirect.com/science/article/pii/S0168945203003777 Shimada et al, 2003



Further information:

Image of Citrus unshiu


Background and prnciples

Fig.2 Reaction catalyzed by ɤ-terpinene synthase.


















Characterization

Western-bloting analysis

(Fig.2 Western-bloting analysis of GST and d-limonene synthase (Lane2), ɤ-terpinene synthase (Lane3) and β-pinene synthase(Lane4) fusion proteins expressed in yeast. (A) The gel picture of SDS-PAGE and western-blot; Lanes: 1, p427-TEF; 2, p427-TEF-LS; 3, p427-TEF-ɤTPNS; 4, p427-TEF-βPINS. (B) vector map of plasmids. (C) The names and molecular weights of each mono-terpene synthase.)

In vivo detection of ɤ-terpinene using GC-MS


Reference


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Unknown