Difference between revisions of "Part:BBa K1445001:Design"

 
 
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===References===
 
===References===
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Jiang, Wenyan, et al. 2013. RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol.

Latest revision as of 03:51, 11 October 2014

Endogenous Type II CRISPR-Cas9 phagemid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2179
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4458
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5400
    Illegal BsaI.rc site found at 5377


Design Notes

We have experienced some toxic effects by the pCas9 part so we suggest growing part-containing cells in lower concentrations of antibiotic, even if plasmid contains a high copy origin of replication.


Source

The M13 origin of replication from Litmus28i and part BBa_K1218011 from the registry.

References

Jiang, Wenyan, et al. 2013. RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol.