Difference between revisions of "Part:BBa K1446003:Experience"

(→‎Applications of BBa_K1446003)
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===Applications of BBa_K1446003===
 
===Applications of BBa_K1446003===
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We cloned this part with an mCherry fusion at the C terminus of the dcmR gene into......<br><br>
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We cloned this part with an mCherry fusion at the C terminus of the dcmR gene in the pOXON-1 plamsid (pME6010 plasmid with kanamycin resistance). Expression was induced with various amounts of ATC and the following fluorescence data acquired. Exposure time was 0.2 seconds. As no calibration data was obtained using purified mCherry, the results have been left in fluorescence arbitrary units.<br>
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The changing mCherry fluorescence shows that the dcmR gene was expressed under the control of the tet promoter and operator system.
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mCherry fluorescence increases with amount of ATC used confirming that the dcmR gene was expressed under the control of the tet promoter and operator system.<br><br>
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Expression was induced with various amounts of ATC and the following fluorescence data acquired. Exposure time was 0.2 seconds. As no calibration data was obtained using purified mCherry, the results have been left in fluorescence arbitrary units.<br><br>
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[[File:Oxford_DcmR-mCherry_expression_induced_by_0ng_ATC.png|centre|750px]]
 
[[File:Oxford_DcmR-mCherry_expression_induced_by_0ng_ATC.png|centre|750px]]
 
[[File:Oxford_DcmR-mCherry_expression_induced_by_10ng_ATC.png|centre|750px]]
 
[[File:Oxford_DcmR-mCherry_expression_induced_by_10ng_ATC.png|centre|750px]]

Revision as of 22:22, 10 October 2014

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Applications of BBa_K1446003

We cloned this part with an mCherry fusion at the C terminus of the dcmR gene in the pOXON-1 plamsid (pME6010 plasmid with kanamycin resistance). Expression was induced with various amounts of ATC and the following fluorescence data acquired. Exposure time was 0.2 seconds. As no calibration data was obtained using purified mCherry, the results have been left in fluorescence arbitrary units.
mCherry fluorescence increases with amount of ATC used confirming that the dcmR gene was expressed under the control of the tet promoter and operator system.

Oxford DcmR-mCherry expression induced by 0ng ATC.png
Oxford DcmR-mCherry expression induced by 10ng ATC.png
Oxford DcmR-mCherry expression induced by 50ng ATC.png
Oxford DcmR-mCherry expression induced by 100ng ATC.png
Oxford DcmR-mCherry expression induced by 200ng ATC.png
Oxford Comparison of mean flouresence intensity from DcmR-mCherry expression induced at varying amounts of ATC.png

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