Difference between revisions of "Part:BBa K1355000"
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The MerA gene is one of the most important gene into bacterial mercury-resistance operon. Transforms mercury Hg2+ in Hg0, which is volatile and moves passively out of the bacteria in gaseous form as shown in Figure above. The reduction of Hg2+ occurs by a NADPH-dependent system. | The MerA gene is one of the most important gene into bacterial mercury-resistance operon. Transforms mercury Hg2+ in Hg0, which is volatile and moves passively out of the bacteria in gaseous form as shown in Figure above. The reduction of Hg2+ occurs by a NADPH-dependent system. | ||
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[[File:l1.png]] | [[File:l1.png]] | ||
Figure 01: Mer genes action in bacterial cell | Figure 01: Mer genes action in bacterial cell | ||
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[[File:l2.png]] | [[File:l2.png]] | ||
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Figure 02: MerA protein 3D Structure | Figure 02: MerA protein 3D Structure | ||
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== Source == | == Source == | ||
Revision as of 20:49, 10 October 2014
Strong RBS + merA (mercuric ion reductase)+ terminator (BBa_ B0015)
The MerA gene is one of the most important gene into bacterial mercury-resistance operon. Transforms mercury Hg2+ in Hg0, which is volatile and moves passively out of the bacteria in gaseous form as shown in Figure above. The reduction of Hg2+ occurs by a NADPH-dependent system.
Figure 01: Mer genes action in bacterial cell
Figure 02: MerA protein 3D Structure
Source
MerA gene sequence is found in the O26-CRL plasmid from Escherichia coli O26. We also added strong RBS from protein 10 found in phage 17 and the double terminator (BBa_B0015) to ensure efficient termination of transcription and messenger RNA recognition.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1200
Illegal NgoMIV site found at 1262 - 1000COMPATIBLE WITH RFC[1000]