Difference between revisions of "Part:BBa K1481006"
 
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<partinfo>BBa_K1481006 short</partinfo>
 
<partinfo>BBa_K1481006 short</partinfo>
  
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R4 recombinase under melibiose promoter for use as a strobe signal for [https://parts.igem.org/Part:BBa_K1481005 BBa_K1481005].
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R4 recombinase under melibiose promoter for use as a strobe signal for [https://parts.igem.org/Part:BBa_K1481005 BBa_K1481005]. It also contains a CDS for transcriptional activator MelR, allowing transcription in presence of melibiose. Note that melibiose transporter activity is temperature-dependent.
  
 
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Latest revision as of 20:00, 10 October 2014

R4 recombinase under melibiose promoter

R4 recombinase under melibiose promoter for use as a strobe signal for BBa_K1481005. It also contains a CDS for transcriptional activator MelR, allowing transcription in presence of melibiose. Note that melibiose transporter activity is temperature-dependent.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1184
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1537
    Illegal NgoMIV site found at 1636
    Illegal NgoMIV site found at 2344
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1835
    Illegal SapI.rc site found at 1258