Difference between revisions of "Part:BBa K1481005"
Line 2: | Line 2: | ||
<partinfo>BBa_K1481005 short</partinfo> | <partinfo>BBa_K1481005 short</partinfo> | ||
− | This device converts result of the AND operation on rhamnose concentration and temperature signals into ampified superfolder GFP expression. It comprises of a rhamnose-inducible promoter pRha with the U10-U RNA thermometer, Bxb1 recombinase, the radB module and the CDS of the reporter protein. | + | This device converts result of the AND operation on rhamnose concentration and temperature signals into ampified superfolder GFP expression. It comprises of a rhamnose-inducible promoter pRha with the U10-U RNA thermometer, Bxb1 recombinase, the radB module and the CDS of the reporter protein. R4 integrase recognition sites allow signal strobing by reporter CDS cleavage. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 19:09, 10 October 2014
Strobable rhamnose AND temperature operated transcriptor SR-latch
This device converts result of the AND operation on rhamnose concentration and temperature signals into ampified superfolder GFP expression. It comprises of a rhamnose-inducible promoter pRha with the U10-U RNA thermometer, Bxb1 recombinase, the radB module and the CDS of the reporter protein. R4 integrase recognition sites allow signal strobing by reporter CDS cleavage.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 355
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 125
Illegal BamHI site found at 629
Illegal XhoI site found at 716 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1924
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1944
Illegal BsaI.rc site found at 1463
Illegal BsaI.rc site found at 1827
Illegal BsaI.rc site found at 2101
Illegal SapI.rc site found at 2193