Difference between revisions of "Part:BBa K1481004"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1481004 short</partinfo>
 
<partinfo>BBa_K1481004 short</partinfo>
  
−
sfGFP under arabinose promoter
+
This part generates a monomeric red fluorescent protein with his-tag for easy protein purification and an MLRR degradation tag. It is controlled by a pBAD arabinose-inducible promoter. It also contains a coding sequence for the AraC protein, crucial for the repression of pBAD in absence of arabinose.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:49, 10 October 2014

sfGFP with MLRR tag under arabinose promoter

This part generates a monomeric red fluorescent protein with his-tag for easy protein purification and an MLRR degradation tag. It is controlled by a pBAD arabinose-inducible promoter. It also contains a coding sequence for the AraC protein, crucial for the repression of pBAD in absence of arabinose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1260