Difference between revisions of "Part:BBa K1362170"

Revision as of 17:14, 10 October 2014

Split sfGFP reconstitution positive control

This part serves as positive control in a split sfGFP intein splicing assay. The N- and C-terminal splicing partners are BBa_K1362173 and BBa_K1362171, the N- and C-terminal non-splicing control halves are BBa_K1362174 and BBa_K1362172, respectively.

The assay was used to both characterize the reconstitution of split superfold GFP (BBa_I746916) as well as to demonstrate the splicing activity of the NpuDnaE split Intein BBa_K1362400. Visit the Heidelberg 2014's [http://2014.igem.org/Project wiki] for a detailed documentation or find a summary of our results in the experience section of this part.

Usage and Biology

This

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 66

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 <partinfo>BBa_K1362170 short</partinfo>
-This part serves as positive control in a split sfGFP intein splicing assay. The N- and C-terminal splicing partners are <partinfo>BBa_K1362171</partinfo> and <partinfo>BBa_K1362173</partinfo>, the non-splicing control halves are <partinfo>BBa_K1362172</partinfo> and <partinfo>BBa_K1362174</partinfo>, respectively.
+This part serves as positive control in a split sfGFP intein splicing assay. The N- and C-terminal splicing partners are <partinfo>BBa_K1362173</partinfo> and <partinfo>BBa_K1362171</partinfo>, the N- and C-terminal non-splicing control halves are <partinfo>BBa_K1362174</partinfo> and <partinfo>BBa_K1362172</partinfo>, respectively.
 The assay was used to both characterize the reconstitution of split superfold GFP (<partinfo>BBa_I746916</partinfo>) as well as to demonstrate the splicing activity of the <i>Npu</i>DnaE split Intein <partinfo>BBa_K1362400</partinfo>.