Difference between revisions of "Part:BBa K1362171"

 
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<partinfo>BBa_K1362171 short</partinfo>
 
<partinfo>BBa_K1362171 short</partinfo>
  
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This part is the N-terminal splicing half in a split sfGFP intein splicing assay. The C-terminal splicing partner is <partinfo>BBa_K1362173</partinfo>, the non-splicing control halves are <partinfo>BBa_K1362172</partinfo> and <partinfo>BBa_K1362174</partinfo>, respectively. <partinfo>BBa_K1362170</partinfo> served as positive control.
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The assay was used to both characterize the reconstitution of split superfold GFP (<partinfo>BBa_I746916</partinfo>) as well as to demonstrate the splicing activity of the <i>Npu</i>DnaE split Intein <partinfo>BBa_K1362400</partinfo>.
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Visit the Heidelberg 2014's [http://2014.igem.org/Project wiki] for a detailed documentation or find a summary of our results in the [[{{PAGENAME}}:Experience|experience section]] of this part.
  
 
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Revision as of 17:10, 10 October 2014

Split sfGFP reconstitution C-terminal splicing half

This part is the N-terminal splicing half in a split sfGFP intein splicing assay. The C-terminal splicing partner is BBa_K1362173, the non-splicing control halves are BBa_K1362172 and BBa_K1362174, respectively. BBa_K1362170 served as positive control.

The assay was used to both characterize the reconstitution of split superfold GFP (BBa_I746916) as well as to demonstrate the splicing activity of the NpuDnaE split Intein BBa_K1362400. Visit the Heidelberg 2014's [http://2014.igem.org/Project wiki] for a detailed documentation or find a summary of our results in the experience section of this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]