Difference between revisions of "Part:BBa K1362170"
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<partinfo>BBa_K1362170 short</partinfo> | <partinfo>BBa_K1362170 short</partinfo> | ||
| − | + | This part serves as positive control in a split sfGFP intein splicing assay. The N- and C-terminal splicing partners are <partinfo>BBa_K1362171</partinfo> and <partinfo>BBa_K1362173</partinfo>, the non-splicing control halves are <partinfo>BBa_K1362172</partinfo> and <partinfo>BBa_K1362174</partinfo>, respectively. | |
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| + | The assay was used to both characterize the reconstitution of split superfold GFP (<partinfo>BBa_I746916</partinfo>) as well as to demonstrate the splicing activity of the <i>Npu</i>DnaE split Intein <partinfo>BBa_K1362400</partinfo>. | ||
| + | Visit the Heidelberg 2014's [http://2014.igem.org/Project wiki] for a detailed documentation or find a summary of our results in the [[{{PAGENAME}}:Experience|experience section]] of this part. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This | ||
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Revision as of 17:06, 10 October 2014
Split sfGFP reconstitution positive control
This part serves as positive control in a split sfGFP intein splicing assay. The N- and C-terminal splicing partners are BBa_K1362171 and BBa_K1362173, the non-splicing control halves are BBa_K1362172 and BBa_K1362174, respectively.
The assay was used to both characterize the reconstitution of split superfold GFP (BBa_I746916) as well as to demonstrate the splicing activity of the NpuDnaE split Intein BBa_K1362400. Visit the Heidelberg 2014's [http://2014.igem.org/Project wiki] for a detailed documentation or find a summary of our results in the experience section of this part.
Usage and Biology
This
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 66