Difference between revisions of "Part:BBa K1477014:Experience"
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| − | According to the Biorad 500 basepair DNA ladder that we used , K1477014 measures at ~2700 base pairs with a pSB1C3 | + | According to the Biorad 500 basepair DNA ladder that we used , K1477014 measures at ~2700 base pairs with a pSB1C3 backbone. This matches our quantitative data.</P> |
http://i.imgur.com/kwgTE7u.png | http://i.imgur.com/kwgTE7u.png | ||
Revision as of 16:16, 10 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1477014
"http://i.imgur.com/FwXzsYX.jpg
According to the Biorad 500 basepair DNA ladder that we used , K1477014 measures at ~2700 base pairs with a pSB1C3 backbone. This matches our quantitative data.
http://i.imgur.com/kwgTE7u.png
We also performed blue/white screening on x-gal plates. When the colonies grew they turned blue, proving that there is Beta-galactosidase activity occurring and that our inserted part is producing the alpha fragment to compliment the omega fragment which naturally occurs in Top 10 cells.
User Reviews
UNIQ38a4bb6dde6af665-partinfo-00000000-QINU UNIQ38a4bb6dde6af665-partinfo-00000001-QINU