Difference between revisions of "Part:BBa K1477014:Experience"
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===Applications of BBa_K1477014=== | ===Applications of BBa_K1477014=== | ||
"http://i.imgur.com/FwXzsYX.jpg | "http://i.imgur.com/FwXzsYX.jpg | ||
| − | According to | + | According to the Biorad 500 basepair DNA ladder that we used , K1477014 measures at ~2600 base pairs with a pSB1C3 bckbone. This matches our quantitative data. |
We also performed blue/white screening on x-gal plates. When the colonies grew they turned blue, proving that there is Beta-galactosidase activity occurring and that our inserted part is producing the alpha fragment to compliment the omega fragment which naturally occurs in Top 10 cells. | We also performed blue/white screening on x-gal plates. When the colonies grew they turned blue, proving that there is Beta-galactosidase activity occurring and that our inserted part is producing the alpha fragment to compliment the omega fragment which naturally occurs in Top 10 cells. | ||
Revision as of 16:05, 10 October 2014
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Applications of BBa_K1477014
"http://i.imgur.com/FwXzsYX.jpg According to the Biorad 500 basepair DNA ladder that we used , K1477014 measures at ~2600 base pairs with a pSB1C3 bckbone. This matches our quantitative data.
We also performed blue/white screening on x-gal plates. When the colonies grew they turned blue, proving that there is Beta-galactosidase activity occurring and that our inserted part is producing the alpha fragment to compliment the omega fragment which naturally occurs in Top 10 cells.
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UNIQc47a06d49e0f1e62-partinfo-00000000-QINU UNIQc47a06d49e0f1e62-partinfo-00000001-QINU