Difference between revisions of "Part:BBa K1431301"

Revision as of 07:26, 10 October 2014

TRE-3G promoter+SV40 PolyA, an ideal controller of mammalian gene expression with Tet-On 3G protein

pTRE-3G promoter is a kind of eukaryocyte cell promoters that will be switched on after connect with a compound which is TetOn protein and tetracycline[TetOn sequence see BBa_K1431101]. That is a very useful way to control whether protein express or not. Of course PolyA is needful in eukaryocyte cell. There are two BbsI sites between promoter and PolyA which can make the coding sequence seamless gather with them. So we can also test how many nucleotides interval between promoter and coding sequence will make the highest expression efficiency.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1431301 short</partinfo>
-pTRE-3G promoter is a kind of eukaryocyte cell promoters that will be switched on after connect with a compound which is TetOn protein and tetracycline[TetOn sequence see BBa_K1431101]. That is a very useful way to control whether protein express or not. Of course PolyA is needful in eukaryocyte cell. There are two BbsI sites between promoter and PolyA which can make the coding sequence seamless gather with them. So we can test how many nucleotides interval between promoter and coding sequence will make the highest expression efficiency.
+pTRE-3G promoter is a kind of eukaryocyte cell promoters that will be switched on after connect with a compound which is TetOn protein and tetracycline[TetOn sequence see BBa_K1431101]. That is a very useful way to control whether protein express or not. Of course PolyA is needful in eukaryocyte cell. There are two BbsI sites between promoter and PolyA which can make the coding sequence seamless gather with them. So we can also test how many nucleotides interval between promoter and coding sequence will make the highest expression efficiency.
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