Difference between revisions of "Part:BBa K1368011"

 
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Spinach Aptamer 13-2 RNA is a small RNA with specific stem loop structure which can combine with DMHBI, a kind of fluorescein, and then the RNA-fluorophore complexes will exhibit fluorescence under the excitation of ultraviolet light. It’s a good substitute for fluorescent protein in the research in transcription level. The endogenous tRNA-lys can be recognized by the cell and avoid being degraded by ribonuclease. We replace the anticodon region of tRNA-lys into Spinach Aptamer coding sequence to make tRNA Scaffold serve as a camouflage, so the Spinach Aptamer is stabilized.
  
 
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Revision as of 04:03, 10 October 2014

Spinach Aptamer 13-2 RNA with Stabilizing tRNA Scaffold

Spinach Aptamer 13-2 RNA is a small RNA with specific stem loop structure which can combine with DMHBI, a kind of fluorescein, and then the RNA-fluorophore complexes will exhibit fluorescence under the excitation of ultraviolet light. It’s a good substitute for fluorescent protein in the research in transcription level. The endogenous tRNA-lys can be recognized by the cell and avoid being degraded by ribonuclease. We replace the anticodon region of tRNA-lys into Spinach Aptamer coding sequence to make tRNA Scaffold serve as a camouflage, so the Spinach Aptamer is stabilized.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]