Difference between revisions of "Part:BBa K1368011"
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− | + | Spinach Aptamer 13-2 RNA is a small RNA with specific stem loop structure which can combine with DMHBI, a kind of fluorescein, and then the RNA-fluorophore complexes will exhibit fluorescence under the excitation of ultraviolet light. It’s a good substitute for fluorescent protein in the research in transcription level. The endogenous tRNA-lys can be recognized by the cell and avoid being degraded by ribonuclease. We replace the anticodon region of tRNA-lys into Spinach Aptamer coding sequence to make tRNA Scaffold serve as a camouflage, so the Spinach Aptamer is stabilized. | |
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Revision as of 04:03, 10 October 2014
Spinach Aptamer 13-2 RNA with Stabilizing tRNA Scaffold
Spinach Aptamer 13-2 RNA is a small RNA with specific stem loop structure which can combine with DMHBI, a kind of fluorescein, and then the RNA-fluorophore complexes will exhibit fluorescence under the excitation of ultraviolet light. It’s a good substitute for fluorescent protein in the research in transcription level. The endogenous tRNA-lys can be recognized by the cell and avoid being degraded by ribonuclease. We replace the anticodon region of tRNA-lys into Spinach Aptamer coding sequence to make tRNA Scaffold serve as a camouflage, so the Spinach Aptamer is stabilized.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]