Difference between revisions of "Part:BBa K1420002"

Revision as of 00:51, 10 October 2014

merB, Organomercurial Lyase from Serratia marcescens

Function

The merB gene is often found immediately downstream of merA, and is essential for the detoxification and bioremediation of organic toxic mercury compounds in congruence with merA. The merB protein is a lyase that catalyzes the breaking of carbon-mercury bonds through protonolysis of toxic mercury compounds, such as methylmercury ("Scheme 1"). This produces the less toxic and less mobile Hg²⁺ which is then completely volatilized to Hg⁰ when acted upon the enzyme merA.

Structure and Mechanism

Enzyme MerB forms a dimer by a 2-fold pseudosymmetry around the depicted rotation axis in "Figure 1". The two subunits are color coded from blue to green distinguishing the amino-terminal end of the protein from the carboxy-terminal, respectively. Active site residues are color coded as well using van der Waals sphere representation: residues Cys-96 and Cys-159 are in yellow, and residue Asp-99 is in red. The structure of mercury-bound MerB and free MerB is extremely similar with the exception of the mercury ion. The mercury is bound to MerB by two sulfurs from the Cys-96 and Cys-159. An oxygen from a water molecule is also involved, binding to the mercury atom. On the other hand, the active site residues for the free MerB are completely inaccessible. MerB can catalyze the protonolysis of carbon mercury bonds for a number of organomercurials, accelerating the rate of this reaction up to 10⁷ times the rate of spontaneous abiotic decay. The proposed mechanism for how this is accomplished is displayed in "Figure 2".

Experimental Results

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 415
1000
COMPATIBLE WITH RFC[1000]

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-<p>The ''merB'' gene is often found immediately downstream of ''merA'', and is essential for the detoxification and bioremediation of organic toxic mercury compounds in congruence with ''merA''. The ''merB'' protein is a lyase that catalyzes the breaking of carbon-mercury bonds through protonolysis of toxic mercury compounds, such as methylmercury. This produces the less toxic and less mobile Hg<sup>2+</sup> which is then completely volatilized to Hg<sup>0</sup> when acted upon the enzyme ''merA''.</p>
+<p>The ''merB'' gene is often found immediately downstream of ''merA'', and is essential for the detoxification and bioremediation of organic toxic mercury compounds in congruence with ''merA''. The ''merB'' protein is a lyase that catalyzes the breaking of carbon-mercury bonds through protonolysis of toxic mercury compounds, such as methylmercury ("Scheme 1"). This produces the less toxic and less mobile Hg<sup>2+</sup> which is then completely volatilized to Hg<sup>0</sup> when acted upon the enzyme ''merA''.</p>
 <b>Structure and Mechanism</b>
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 [[File:MerB_mechanism2.JPG‎ ]]
 <p></p>
-<p>Enzyme MerB forms a dimer by a 2-fold pseudosymmetry around the depicted rotation axis in Figure 1. The two subunits are color coded from blue to green distinguishing the amino-terminal end of the protein from the carboxy-terminal, respectively. Active site residues are color coded as well using van der Waals sphere representation: residues Cys-96 and Cys-159 are in yellow, and residue Asp-99 is in red. The structure of mercury-bound MerB and free MerB is extremely similar with the exception of the mercury ion. The mercury is bound to MerB by two sulfurs from the Cys-96 and Cys-159. An oxygen from a water molecule is also involved, binding to the mercury as well as the two sulfurs from the Cys residues. On the other hand, the active site residues for the free MerB are completely inaccessible.
+<p>Enzyme MerB forms a dimer by a 2-fold pseudosymmetry around the depicted rotation axis in "Figure 1". The two subunits are color coded from blue to green distinguishing the amino-terminal end of the protein from the carboxy-terminal, respectively. Active site residues are color coded as well using van der Waals sphere representation: residues Cys-96 and Cys-159 are in yellow, and residue Asp-99 is in red. The structure of mercury-bound MerB and free MerB is extremely similar with the exception of the mercury ion. The mercury is bound to MerB by two sulfurs from the Cys-96 and Cys-159. An oxygen from a water molecule is also involved, binding to the mercury atom. On the other hand, the active site residues for the free MerB are completely inaccessible.
-</p>
+MerB can catalyze the protonolysis of  carbon mercury bonds for a number of organomercurials, accelerating the rate of this reaction up to 10<sup>7</sup> times the rate of spontaneous abiotic decay. The proposed mechanism for how this is accomplished is displayed in "Figure 2".</p>
 <b>Experimental Results</b>
 <b></b>