Difference between revisions of "Part:BBa K1362439"
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<partinfo>BBa_K1362439 short</partinfo> | <partinfo>BBa_K1362439 short</partinfo> | ||
| − | + | This linker with the amino acid sequence GGAEAAAKEAAAKAAP was used to circularize λ-Lysozyme. The purpose of this linker is to achieve maximal thermostability, while preserving the native 3D structure of the protein. Our model [[http://2014.igem.org/Team:Heidelberg/Modeling/Linker_Modeling]] is based on the assumption that linkers have to be as short and rigid as possible in order to allow minimal conformational changes upon heating. On the other hand, linkers for circularization should be long enough not to cause conformational strain. This could impact both protein function and the cyclization reaction. | |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 22:39, 9 October 2014
rigid linker for λ-Lysozyme
This linker with the amino acid sequence GGAEAAAKEAAAKAAP was used to circularize λ-Lysozyme. The purpose of this linker is to achieve maximal thermostability, while preserving the native 3D structure of the protein. Our model http://2014.igem.org/Team:Heidelberg/Modeling/Linker_Modeling is based on the assumption that linkers have to be as short and rigid as possible in order to allow minimal conformational changes upon heating. On the other hand, linkers for circularization should be long enough not to cause conformational strain. This could impact both protein function and the cyclization reaction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]