Difference between revisions of "Part:BBa K1362439:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1362439 short</partinfo> | <partinfo>BBa_K1362439 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | This Linker has the amino acid sequence GGAEAAAKEAAAKAAP. AEAAAKEAAAKA [[#References|[1]]] forms a rigid alpha helix. GG is a flexible spacer sequence. The P was added to make sure that the helix is disrupted. | |
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===Source=== | ===Source=== | ||
| − | + | This sequences was obtained by DNA synthesis. | |
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===References=== | ===References=== | ||
| + | [1] Arai, R., Ueda, H., Kitayama, a, Kamiya, N. & Nagamune, T. Design of the linkers which effectively separate domains of a bifunctional fusion protein. Protein Eng. 14, 529–32 (2001). | ||
Latest revision as of 22:11, 9 October 2014
rigid linker for λ-Lysozyme
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This Linker has the amino acid sequence GGAEAAAKEAAAKAAP. AEAAAKEAAAKA [1] forms a rigid alpha helix. GG is a flexible spacer sequence. The P was added to make sure that the helix is disrupted.
Source
This sequences was obtained by DNA synthesis.
References
[1] Arai, R., Ueda, H., Kitayama, a, Kamiya, N. & Nagamune, T. Design of the linkers which effectively separate domains of a bifunctional fusion protein. Protein Eng. 14, 529–32 (2001).