Difference between revisions of "Part:BBa K1446003:Experience"
(→Applications of BBa_K1446003) |
|||
| Line 5: | Line 5: | ||
===Applications of BBa_K1446003=== | ===Applications of BBa_K1446003=== | ||
We cloned this part with an mCherry fusion at the C terminus of the dcmR gene into......<br><br> | We cloned this part with an mCherry fusion at the C terminus of the dcmR gene into......<br><br> | ||
| − | The changing mCherry | + | The changing mCherry fluorescence shows that the dcmR gene was expressed under the control of the tet promoter and operator system. |
Expression was induced with various amounts of ATC and the following fluorescence data acquired. Exposure time was 0.2 seconds. As no calibration data was obtained using purified mCherry, the results have been left in fluorescence arbitrary units.<br><br> | Expression was induced with various amounts of ATC and the following fluorescence data acquired. Exposure time was 0.2 seconds. As no calibration data was obtained using purified mCherry, the results have been left in fluorescence arbitrary units.<br><br> | ||
[[File:Oxford_DcmR-mCherry_expression_induced_by_0ng_ATC.png|centre|750px]] | [[File:Oxford_DcmR-mCherry_expression_induced_by_0ng_ATC.png|centre|750px]] | ||
| Line 13: | Line 13: | ||
[[File:Oxford_DcmR-mCherry_expression_induced_by_200ng_ATC.png|centre|750px]] | [[File:Oxford_DcmR-mCherry_expression_induced_by_200ng_ATC.png|centre|750px]] | ||
[[File:Oxford_Comparison_of_mean_flouresence_intensity_from_DcmR-mCherry_expression_induced_at_varying_amounts_of_ATC.png|centre|900px]] | [[File:Oxford_Comparison_of_mean_flouresence_intensity_from_DcmR-mCherry_expression_induced_at_varying_amounts_of_ATC.png|centre|900px]] | ||
| + | |||
===User Reviews=== | ===User Reviews=== | ||
<!-- DON'T DELETE --><partinfo>BBa_K1446003 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K1446003 StartReviews</partinfo> | ||
Revision as of 22:11, 9 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1446003
We cloned this part with an mCherry fusion at the C terminus of the dcmR gene into......
The changing mCherry fluorescence shows that the dcmR gene was expressed under the control of the tet promoter and operator system.
Expression was induced with various amounts of ATC and the following fluorescence data acquired. Exposure time was 0.2 seconds. As no calibration data was obtained using purified mCherry, the results have been left in fluorescence arbitrary units.
User Reviews
UNIQ31618ece96236eb1-partinfo-00000000-QINU UNIQ31618ece96236eb1-partinfo-00000001-QINU