Difference between revisions of "Part:BBa K1497019"
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<partinfo>BBa_K1497019 short</partinfo>
 
<partinfo>BBa_K1497019 short</partinfo>
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FdeR is a homo dimeric protein from ''Herbaspirillum seropedicae''. In present of naringenin, FdeR activates the specific promotor region upstream of the fdeR region and allow a strong gene expression.
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We modified the naringenin biosensor from Siedler et al. by elimination of the internal EcoRI restriction site and replaced the CFP by the Biobrick Suffix. Based on this reporter protein and 3 different fluorescence proteins, we designed 3 new biosensors for in vivo detection and determination of naringenin.
  
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fdeR - Naringenin binding protein
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[[File:Homology_model_of_FdeR.png|300px|thumb|right|Homology model of FdeR. PDB: 2ESN was used as template. ]]
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We modified the naringenin biosensor from Siedler et al. by elimination of one EcoRI restriction site and by changing the native ribosomal binding site into the strong RBS Bba_B0034. Based on this reporter protein and 3 different fluorescence proteins, we designed 3 new biosensors for in vivo detection and determination of naringenin. The natural source of FdeR is Herbaspirillum seropedicae.
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You can use our reporter for measuring naringenin concentrations in your samples. Depending on which fluorophor you want to detect, you can use one of three biosensors:
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GFP  K1497020
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===Usage and Biology==
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mKate (DsRed variant) K1497021
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CFP K1497022
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You can create your own naringenin sensor or your own naringenin dependent gene expression device as well. For these reasons use FdeR Biobrick K1497019 and clone your parts of interest (without RBS!) behind the device.
 
You can create your own naringenin sensor or your own naringenin dependent gene expression device as well. For these reasons use FdeR Biobrick K1497019 and clone your parts of interest (without RBS!) behind the device.
 +
 +
 +
You can use the reporters for measuring naringenin concentrations in your samples.
 +
Depending on which fluorophor you want to detect, you can use one of three biosensors:
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<html>
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<BLOCKQUOTE>
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    <li>GFP  K1497020</li>
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    <li>mKate K1497021</li>
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    <li>CFP K1497022</li>
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</BLOCKQUOTE>
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</html>
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Revision as of 21:31, 9 October 2014

fdeR (Naringenin binding protein) and fde operon regulatory domain FdeR is a homo dimeric protein from Herbaspirillum seropedicae. In present of naringenin, FdeR activates the specific promotor region upstream of the fdeR region and allow a strong gene expression.

We modified the naringenin biosensor from Siedler et al. by elimination of the internal EcoRI restriction site and replaced the CFP by the Biobrick Suffix. Based on this reporter protein and 3 different fluorescence proteins, we designed 3 new biosensors for in vivo detection and determination of naringenin.

Homology model of FdeR. PDB: 2ESN was used as template.


=Usage and Biology

You can create your own naringenin sensor or your own naringenin dependent gene expression device as well. For these reasons use FdeR Biobrick K1497019 and clone your parts of interest (without RBS!) behind the device.


You can use the reporters for measuring naringenin concentrations in your samples. Depending on which fluorophor you want to detect, you can use one of three biosensors:


  • GFP K1497020
  • mKate K1497021
  • CFP K1497022



    • Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      COMPATIBLE WITH RFC[12]
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      INCOMPATIBLE WITH RFC[25]
      Illegal NgoMIV site found at 95
      Illegal NgoMIV site found at 452
      Illegal NgoMIV site found at 543
      Illegal NgoMIV site found at 555
    • 1000
      INCOMPATIBLE WITH RFC[1000]
      Illegal BsaI.rc site found at 271