Difference between revisions of "Part:BBa K1321301"
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<partinfo>BBa_K1321301 short</partinfo>
 
<partinfo>BBa_K1321301 short</partinfo>
  
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This is pSEVA321 converted into a biobrick compatible format by substituting the original multiple cloning site for biobrick prefix and suffix. pSEVA321 is a broad host range plasmid, capable of replication in E.coli and several other bacterial species. We have verified that it also capable of replication in the cellulose producing bacterium Gluconacetobacter xylinus. We have used it as a backbone for G.xylinus genetic engineering.  
+
This is pSEVA321 converted into a biobrick compatible format by substituting the original multiple cloning site for biobrick prefix and suffix. pSEVA321 is a broad host range plasmid, capable of replication in E.coli and several other bacterial species. We have verified that it also capable of replication in the cellulose producing bacterium Gluconacetobacter xylinus. We have used it as a shuttle vector for G.xylinus genetic engineering.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:52, 9 October 2014

pSEVA321-Bb

This is pSEVA321 converted into a biobrick compatible format by substituting the original multiple cloning site for biobrick prefix and suffix. pSEVA321 is a broad host range plasmid, capable of replication in E.coli and several other bacterial species. We have verified that it also capable of replication in the cellulose producing bacterium Gluconacetobacter xylinus. We have used it as a shuttle vector for G.xylinus genetic engineering.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3572
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3578
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3572
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3572
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3572
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3587
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1191
    Illegal NgoMIV site found at 2076
    Illegal NgoMIV site found at 3187
    Illegal NgoMIV site found at 3311
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.