Difference between revisions of "Part:BBa K1412007"
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=='''Method'''==
 
=='''Method'''==
  
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Contructing new device by this part, then characterize the device in <i>E.coli CL-1</i>(knock <i>CheZ</i> gene out of genome). In order for reference, we need to choose appropriate reference parts(see the wiki from iGEM14_XMU-China) which has only one portion(RBS or promoter) different from the new contructed device. As the expression strength from reference part is already known, we can spot both kinds of device on the same semi-solid medium culture. By comparing the chemotaxis diameter of both devices, we can tell unknown expression strength of unknown promoters or RBSs.
+
Contructing new device by this part, then characterize the device in <i>E.coli CL-1</i>(knock <i>CheZ</i> gene out of genome). In order to take reference, we need to choose appropriate reference parts(see the wiki from iGEM14_XMU-China) which has only one portion(RBS or promoter) different from the new contructed device. As the expression strength from reference part is already known, we can spot both kinds of device on the same semi-solid medium culture. By comparing the chemotaxis diameter of both devices, we can tell unknown expression strength of unknown promoters or RBSs.
  
  

Revision as of 16:13, 9 October 2014

CheZ-TT

This part is derived from BBa_K1412005 by taking RBS out.

As iGEM14_XMU-China has developed a system to characterize the strength of promoters and RBSs, this part provides an easier assembly path to contruct new devices to characterize the strength of promoters and RBSs which is unknown.


Method

Contructing new device by this part, then characterize the device in E.coli CL-1(knock CheZ gene out of genome). In order to take reference, we need to choose appropriate reference parts(see the wiki from iGEM14_XMU-China) which has only one portion(RBS or promoter) different from the new contructed device. As the expression strength from reference part is already known, we can spot both kinds of device on the same semi-solid medium culture. By comparing the chemotaxis diameter of both devices, we can tell unknown expression strength of unknown promoters or RBSs.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1] [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0011915#pone-0011915-g006 Paungfoo-Lonhienne C et al. (2010) Turning the table: plants consume microbes as a source of nutrients. PLoS One 5(7): e11915 ].

[2] [http://pubs.acs.org/doi/abs/10.1021/bi00561a015 Chelsky D and Dahlquist FW (1980) Chemotaxis in Escherichia coli: association of protein components. Biochemistry 19: 4633–4639 ].