Difference between revisions of "Part:BBa K1349006:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The sequence was mutated by site-directed mutagenesis to remove the EcoRI site.
 
  
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This part is the CheA histidine Kinase that forms part of the Chemotaxis system in E. coli. Naturally this protein is able to auto-phosphorylate and transfer the phosphate groups to CheB and CheY.
  
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Obtained by PCR from E.coli and SLIC assembly. The construction removed the EcoR1 restriction site in the gene by silent mutations of ATT codon to a ATC codon. the construction contains the entire coding sequence.
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Expression of this part is expected to complement the chemotaxis minus phenotype of a CheA mutant.
  
 
===Source===
 
===Source===

Revision as of 07:47, 9 October 2014


cheA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1636
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is the CheA histidine Kinase that forms part of the Chemotaxis system in E. coli. Naturally this protein is able to auto-phosphorylate and transfer the phosphate groups to CheB and CheY.

Obtained by PCR from E.coli and SLIC assembly. The construction removed the EcoR1 restriction site in the gene by silent mutations of ATT codon to a ATC codon. the construction contains the entire coding sequence.

Expression of this part is expected to complement the chemotaxis minus phenotype of a CheA mutant.

Source

xxx

References