Difference between revisions of "Part:BBa K1349003:Design"
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===References=== | ===References=== | ||
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| + | Whitaker WR, Davis SA, Arkin AP, Dueber JE. 2012. | ||
| + | Engineering robust control of two-component system phosphotransfer using modular scaffolds. | ||
| + | ''Proc Natl Acad Sci U S A.'' '''109(44):'''18090-5. doi: 10.1073/pnas.1209230109. | ||
Latest revision as of 07:36, 9 October 2014
PcusC
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
CusR is a response regulator that binds to DNA when phosphorylated. This small part is the sensitive CusR promoter from E. coli and we designed it to be a promoter positively regulated by CusR phosphorylation. In common with many response regulators we expect over-expression of CusR to result in binding and so positive regulation of the promoter. This part is complementary to part BBa_K1349002.
A construct with this part downstream of a gene is expected to show positive regulation of the gene by CusR phosphorylation (or CusR overexpression).
Source
The part was amplified by PCR from the pWW2181 plasmid described by Whitaker et al.
References
Whitaker WR, Davis SA, Arkin AP, Dueber JE. 2012. Engineering robust control of two-component system phosphotransfer using modular scaffolds. Proc Natl Acad Sci U S A. 109(44):18090-5. doi: 10.1073/pnas.1209230109.