Difference between revisions of "Part:BBa K1349003:Design"

 
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===Design Notes===
 
===Design Notes===
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CusR is a response regulator that binds to DNA when phosphorylated. This small part is the sensitive CusR promoter from E. coli and we designed it to be a promoter positively regulated by CusR phosphorylation. In common with many response regulators we expect over-expression of CusR to result in binding and so positive regulation of the promoter.  This part  is complementary to part BBa_K1349002.
  
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A construct with this part downstream of a gene is expected to show positive regulation of the gene by CusR phosphorylation (or CusR overexpression).
  
 
===Source===
 
===Source===

Revision as of 07:32, 9 October 2014


PcusC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CusR is a response regulator that binds to DNA when phosphorylated. This small part is the sensitive CusR promoter from E. coli and we designed it to be a promoter positively regulated by CusR phosphorylation. In common with many response regulators we expect over-expression of CusR to result in binding and so positive regulation of the promoter. This part is complementary to part BBa_K1349002.

A construct with this part downstream of a gene is expected to show positive regulation of the gene by CusR phosphorylation (or CusR overexpression).

Source

xxx

References