Difference between revisions of "Part:BBa K1465228:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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BB
 
  
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*Amplification of the pSB1C3-Backbone (<bbpart>BBa_J04450</bbpart>) using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw-pSB1C3-BBa_B0034-SBPase fw-pSB1C3-BBa_B0034-SBPase] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rv-SBPase-BBa_B0034-pSB1C3 rv-SBPase-BBa_B0034-pSB1C3]:<br>
  
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'''fw-pSB1C3-BBa_B0034-SBPase (58 bp)'''<br>
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5'-GAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGAGGGAATTGAAAAGCGAAAAG-3'<br>
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'''rv-SBPase-BBa_B0034-pSB1C3 (65 bp)'''<br>
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5'-TTTTCGCTTTTCAATTCCCTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCCAGAAATC-3'<br>
  
 
===Source===
 
===Source===

Revision as of 23:10, 8 October 2014


Sedoheptulose 1,7-bisphosphatase (glpX) from Bacillus methanolicus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Amplification of the pSB1C3-Backbone (BBa_J04450) using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw-pSB1C3-BBa_B0034-SBPase fw-pSB1C3-BBa_B0034-SBPase] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rv-SBPase-BBa_B0034-pSB1C3 rv-SBPase-BBa_B0034-pSB1C3]:
fw-pSB1C3-BBa_B0034-SBPase (58 bp)

5'-GAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGAGGGAATTGAAAAGCGAAAAG-3'

rv-SBPase-BBa_B0034-pSB1C3 (65 bp)

5'-TTTTCGCTTTTCAATTCCCTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCCAGAAATC-3'

Source

Gibson

References