Difference between revisions of "Part:BBa K1362443:Design"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1362443 short</partinfo> | <partinfo>BBa_K1362443 short</partinfo> | ||
| Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | Well, its just a his6 tag. | |
| − | + | ||
===Source=== | ===Source=== | ||
| + | The amino acid sequence HHHHHH [[#References|[1]]] was backtranslated and obtained via DNA oligo synthesis. | ||
| − | |||
===References=== | ===References=== | ||
| + | [1] Hochuli, E.; Bannwarth, W.; Döbeli, H.; Gentz, R.; Stüber, D. (1988). "Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent". Nature Biotechnology 6 (11): 1321–1326 | ||
Latest revision as of 22:19, 8 October 2014
His6 linker for Xylanase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Well, its just a his6 tag.
Source
The amino acid sequence HHHHHH [1] was backtranslated and obtained via DNA oligo synthesis.
References
[1] Hochuli, E.; Bannwarth, W.; Döbeli, H.; Gentz, R.; Stüber, D. (1988). "Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent". Nature Biotechnology 6 (11): 1321–1326