Difference between revisions of "Part:BBa K1362442:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1362442 short</partinfo> | <partinfo>BBa_K1362442 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The his6 tag serves as an epitope tag. The GS spacers were added just for fun. | |
| − | + | ||
===Source=== | ===Source=== | ||
| + | Short GS linker sequences were added to the amino acid sequence HHHHHH [[#References|[1]]]. GSHHHHHHSG was backtranslated and obtained via DNA oligo synthesis. | ||
| − | |||
===References=== | ===References=== | ||
| + | [1] Hochuli, E.; Bannwarth, W.; Döbeli, H.; Gentz, R.; Stüber, D. (1988). "Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent". Nature Biotechnology 6 (11): 1321–1325 | ||
Latest revision as of 22:17, 8 October 2014
His6 tag with spacers
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The his6 tag serves as an epitope tag. The GS spacers were added just for fun.
Source
Short GS linker sequences were added to the amino acid sequence HHHHHH [1]. GSHHHHHHSG was backtranslated and obtained via DNA oligo synthesis.
References
[1] Hochuli, E.; Bannwarth, W.; Döbeli, H.; Gentz, R.; Stüber, D. (1988). "Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent". Nature Biotechnology 6 (11): 1321–1325