Difference between revisions of "Part:BBa K1362435:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The his6 tag [[#References|[1]]] serves as an epitope tag. The GS spacers were added to adjust the length of the linker. | |
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===Source=== | ===Source=== | ||
| + | Short GS linker sequences were added to the amino acid sequence HHHHHH [[#References|[1]]]. GSHHHHHHSG backtranslated and obtained via DNA oligo synthesis. | ||
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===References=== | ===References=== | ||
| + | [1] Hochuli, E.; Bannwarth, W.; Döbeli, H.; Gentz, R.; Stüber, D. (1988). "Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent". Nature Biotechnology 6 (11): 1321–1325 | ||
Latest revision as of 22:08, 8 October 2014
His6 linker for λ-Lysozyme
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The his6 tag [1] serves as an epitope tag. The GS spacers were added to adjust the length of the linker.
Source
Short GS linker sequences were added to the amino acid sequence HHHHHH [1]. GSHHHHHHSG backtranslated and obtained via DNA oligo synthesis.
References
[1] Hochuli, E.; Bannwarth, W.; Döbeli, H.; Gentz, R.; Stüber, D. (1988). "Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent". Nature Biotechnology 6 (11): 1321–1325