Difference between revisions of "Part:BBa K1362433:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1362433 short</partinfo> | <partinfo>BBa_K1362433 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Based on a large-scale analysis of exteins [[#References|[1]]], the C-extein CWE and the associated N-extein RGK were discovered to be most efficient. | |
===Source=== | ===Source=== | ||
| − | + | The amino acid sequence CWE [[#References|[1]]] was backtranslated and obtained via DNA oligo synthesis. | |
| − | + | ||
===References=== | ===References=== | ||
| + | [1] Cheriyan M., Pedamallu C. S., Tori K., Perler F. (2013) Faster protein splicing with the Nostoc punctiforme DnaE intein using non-native extein residues. J. Biol. Chem. 288, 6202–6211 | ||
Latest revision as of 21:49, 8 October 2014
CWE (extein)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Based on a large-scale analysis of exteins [1], the C-extein CWE and the associated N-extein RGK were discovered to be most efficient.
Source
The amino acid sequence CWE [1] was backtranslated and obtained via DNA oligo synthesis.
References
[1] Cheriyan M., Pedamallu C. S., Tori K., Perler F. (2013) Faster protein splicing with the Nostoc punctiforme DnaE intein using non-native extein residues. J. Biol. Chem. 288, 6202–6211