Difference between revisions of "Part:BBa K1362433"

 
 
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<partinfo>BBa_K1362433 short</partinfo>
 
<partinfo>BBa_K1362433 short</partinfo>
  
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This sequence is coding for the amino acid sequence CWE. CWE is an improved non-native C-extein of the intein NpuDnaE. The associated N-extein is RGK (<partinfo>BBa_K1362434</partinfo>). Information about inteins is provided here: <partinfo>BBa_K1362400</partinfo>.
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Exteins are the amino acids next to an intein. They have a great impact on splicing efficiency. Thus, good exteins are usually added between the protein of interest and inteins. However, they will remain as scars after splicing.
  
 
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Latest revision as of 21:47, 8 October 2014

CWE (extein)

This sequence is coding for the amino acid sequence CWE. CWE is an improved non-native C-extein of the intein NpuDnaE. The associated N-extein is RGK (BBa_K1362434). Information about inteins is provided here: BBa_K1362400.

Exteins are the amino acids next to an intein. They have a great impact on splicing efficiency. Thus, good exteins are usually added between the protein of interest and inteins. However, they will remain as scars after splicing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]