Difference between revisions of "Part:BBa K1362102"
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<partinfo>BBa_K1362102 short</partinfo> | <partinfo>BBa_K1362102 short</partinfo> | ||
| − | + | This NpuDnaE(N*) intein assembly construct can serve as a control for the splicing reaction. The N-terminal cystein residue was mutated to a glycine to prevent the nucleophilic attack which initiates protein splicing. Read more about the intein assembly construct in <partinfo>BBa_K1362100</partinfo>. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 20:25, 8 October 2014
RBS + NpuDnaE N-intein RFC[105] non-splicing control construct (with His6)
This NpuDnaE(N*) intein assembly construct can serve as a control for the splicing reaction. The N-terminal cystein residue was mutated to a glycine to prevent the nucleophilic attack which initiates protein splicing. Read more about the intein assembly construct in BBa_K1362100. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 826
Illegal AgeI site found at 938 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1115
Illegal BsaI.rc site found at 40