Difference between revisions of "Part:BBa J33201:Experience"

Revision as of 16:05, 13 October 2006

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J33201

This part is designed for the detection of arsenic (as arsenate or arsenite) in drinking water. Various reporter genes can be attached to the part and will be induced in the presence of arsenate or arsenite.

User Reviews

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+As part of IGEM 2006, the Edinburgh team attached this part to a lacZ' reporter gene and observed activity in the presence of arsenate. When lactose was included in the growth medium, lactose hydrolysis by LacZ, followed by lactose fermentation, resulted in a considerable pH drop in the presence of arsenate at concentrations as low as 5 ppb arsenic (0.067 micromolar). Effects typically required around 5 hours incubation in the presence of arsenate to become manifest, longer at arsenate concentrations below about 0.2 micromolar. In other work using xylE as a reporter gene, we have observed a similar time course, with around 5 hours induction required for an obervable increase in reporter gene activity.
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