Difference between revisions of "Part:BBa K1346006"
 
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Compared to other alpha factor responsive promoters, pPRM3 has a low basal expression level as well as low levels of output.
 
Compared to other alpha factor responsive promoters, pPRM3 has a low basal expression level as well as low levels of output.
  
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File:140627 AFRP GFP ROUND 3.png|900px]]
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[[File:140627 AFRP GFP ROUND 3.png|900px]]
  
  

Latest revision as of 18:52, 8 October 2014

pPRM3

Promoter endogenous to s. cerevisiae that is induced by alpha factor. Characterized on flow cytometer by measuring its mean GFP fluorescence over 8 concentrations of alpha factor.

PRM3.png

Compared to other alpha factor responsive promoters, pPRM3 has a low basal expression level as well as low levels of output.

140627 AFRP GFP ROUND 3.png


Alpha factor responsive promoters were selected from the following paper: Roberts, Christopher J., et al (2000 Feb 4). Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles. Science, 287(5454):873-80. http://www.ncbi.nlm.nih.gov/pubmed/10657304.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 71
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]