Difference between revisions of "Part:BBa K1346006"
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Promoter endogenous to s. cerevisiae that is induced by alpha factor. Characterized on flow cytometer by measuring its mean GFP fluorescence over 8 concentrations of alpha factor. | Promoter endogenous to s. cerevisiae that is induced by alpha factor. Characterized on flow cytometer by measuring its mean GFP fluorescence over 8 concentrations of alpha factor. | ||
| + | [[File:PRM3.png|900px]] | ||
| + | Compared to other alpha factor responsive promoters, pPRM3 has a low basal expression level as well as low levels of output. | ||
| + | File:140627 AFRP GFP ROUND 3.png|900px]] | ||
Revision as of 18:51, 8 October 2014
pPRM3
Promoter endogenous to s. cerevisiae that is induced by alpha factor. Characterized on flow cytometer by measuring its mean GFP fluorescence over 8 concentrations of alpha factor.
Compared to other alpha factor responsive promoters, pPRM3 has a low basal expression level as well as low levels of output.
File:140627 AFRP GFP ROUND 3.png|900px]]
Alpha factor responsive promoters were selected from the following paper: Roberts, Christopher J., et al (2000 Feb 4). Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles. Science, 287(5454):873-80. http://www.ncbi.nlm.nih.gov/pubmed/10657304.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 71
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]