Difference between revisions of "Part:BBa K563004:Experience"

 
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<partinfo>BBa_K431008 AddReview 4</partinfo>
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<I>UCSF-UCB iGEM 2014</I>
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We used pTEF1 in pSV606 (S. cerevisiae) yeast strains to create a constitutive circuit for our project, and it performed consistently as a constitutive promoter.
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We characterized pTEF1 alongside some of its mutants (m3, m6, m7, and m10), as seen in the following graph. If one would like to optimize expressiveness, pTEFm7 was found to have a higher level of constitutiveness than pTEF1, but pTEF1 is still a reliable promoter.
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[[File:140630_pTEF1_GFP_ROUND_1.png|800px]]
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The mutants were selected from a 2006 paper by Elke Nevoigt and Jessica Kohnke: http://www.ncbi.nlm.nih.gov/pubmed/16885275.
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pTEF1 is also listed as parts BBa_K431008 and BBa_K431008.
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Revision as of 18:24, 8 October 2014


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K563004

User Reviews

UNIQe8bd04b1923b1be7-partinfo-00000000-QINU UNIQe8bd04b1923b1be7-partinfo-00000001-QINU

UNIQe8bd04b1923b1be7-partinfo-00000002-QINU

••••

UCSF-UCB iGEM 2014

We used pTEF1 in pSV606 (S. cerevisiae) yeast strains to create a constitutive circuit for our project, and it performed consistently as a constitutive promoter. We characterized pTEF1 alongside some of its mutants (m3, m6, m7, and m10), as seen in the following graph. If one would like to optimize expressiveness, pTEFm7 was found to have a higher level of constitutiveness than pTEF1, but pTEF1 is still a reliable promoter.

140630 pTEF1 GFP ROUND 1.png

The mutants were selected from a 2006 paper by Elke Nevoigt and Jessica Kohnke: http://www.ncbi.nlm.nih.gov/pubmed/16885275.

pTEF1 is also listed as parts BBa_K431008 and BBa_K431008.

;