Difference between revisions of "Part:BBa K1362100:Design"
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===Design Notes=== | ===Design Notes=== | ||
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+ | The part is designed in compliance with RFC[???] [#References|[1]]. For an exact description of how to use it or how to design other intein constructs accordingly, please read the iGEM team Heidelberg 2014's [http://2014.igem.org/Team:Heidelberg/parts/rfc wikipage] or the RFC[???]. | ||
===Source=== | ===Source=== |
Revision as of 17:25, 8 October 2014
RBS + NpuDnaE N-intein RFC[105] assembly construct (with His6)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 826
Illegal AgeI site found at 938 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1115
Illegal BsaI.rc site found at 40
Design Notes
The part is designed in compliance with RFC[???] [#References|[1]]. For an exact description of how to use it or how to design other intein constructs accordingly, please read the iGEM team Heidelberg 2014's [http://2014.igem.org/Team:Heidelberg/parts/rfc wikipage] or the RFC[???].
Source
The part is assembled from different sources. The intein sequences come addgene or were kindly provided by different working groups. BBa_J04450 was used as mRFP selection marker. The scars and overhangs are part of the RFC[???] intein cloning strategy and are further explained on our [http://2014.igem.org/Team:Heidelberg/parts/rfc wiki] and in RFC[???] [1]. Please see the corresponding basic parts for the individual detailed information.
References
[1] [link RFC[???]]