Difference between revisions of "Part:BBa K1497019"

Revision as of 16:42, 8 October 2014

fdeR (Naringenin binding protein) and fde operon regulatory domain

fdeR - Naringenin binding protein We modified the naringenin biosensor from Siedler et al. by elimination of one EcoRI restriction site and by changing the native ribosomal binding site into the strong RBS Bba_B0034. Based on this reporter protein and 3 different fluorescence proteins, we designed 3 new biosensors for in vivo detection and determination of naringenin. The natural source of FdeR is Herbaspirillum seropedicae. You can use our reporter for measuring naringenin concentrations in your samples. Depending on which fluorophor you want to detect, you can use one of three biosensors: GFP K1497020 mKate (DsRed variant) K1497021 CFP K1497022

You can create your own naringenin sensor or your own naringenin dependent gene expression device as well. For these reasons use FdeR Biobrick K1497019 and clone your parts of interest (without RBS!) behind the device.

Sequence and Features