Difference between revisions of "Part:BBa K1455003"
 
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<partinfo>BBa_K1455003 short</partinfo>
 
<partinfo>BBa_K1455003 short</partinfo>
  
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luxO D47E gene followed by GFP reporter.  
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mRFP chromoprotein under tetracylcine-repressive promoter.
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Bacteria with this device are red due to the mRFP constitutive expression. This espression turns off if TetR repressor is present and it can be restored by the addition of tetracycline or analogues (as anhydrotetracycline).
  
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This device can be assembled with any promoter to obatin any kind of expression for LusOD 47E and to measure it by fluorescence of GFP as was made in [https://parts.igem.org/Part:BBa_K1455002 BBa_K1455002].
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Here is an example of E. coli DH5α carrying this device:
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LuxOD 47E acts like a phosphorilated LuxO, which is an essential protein of Vibrio spp. quorum sensing system. LuxO is the final protein in a phophorilation cascade and binds, as a trancription facotr, to pqrr promoters. LuxOD 47E is able to activate the genes downstream of a pqrr promoter.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:24, 8 October 2014

pTet - mRFP

mRFP chromoprotein under tetracylcine-repressive promoter. Bacteria with this device are red due to the mRFP constitutive expression. This espression turns off if TetR repressor is present and it can be restored by the addition of tetracycline or analogues (as anhydrotetracycline).

Here is an example of E. coli DH5α carrying this device:

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 635
    Illegal AgeI site found at 747
  • 1000
    COMPATIBLE WITH RFC[1000]