Difference between revisions of "Part:BBa K1424003"
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The kanamycin gene to allow transformation in Synechocystis sp. PCC 6803 (Poteete et al, 2006). | The kanamycin gene to allow transformation in Synechocystis sp. PCC 6803 (Poteete et al, 2006). | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
ADVANTAGES IN GENOME ENGINEERING APPLICATIONS: | ADVANTAGES IN GENOME ENGINEERING APPLICATIONS: | ||
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===Improved Function=== | ===Improved Function=== | ||
This part is an improvement to the documented part BBa_K300989. It has been optimized for use in the organism Synechocystis sp. PCC 6803, and also optimized to be compatible with iGEM assembly. | This part is an improvement to the documented part BBa_K300989. It has been optimized for use in the organism Synechocystis sp. PCC 6803, and also optimized to be compatible with iGEM assembly. | ||
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Revision as of 09:35, 8 October 2014
Kanamycin resistance for Synechocystis
The kanamycin resistance gene tn903 aph from E. coli for use with Synechocystis sp. PCC 6803.
The kanamycin gene to allow transformation in Synechocystis sp. PCC 6803 (Poteete et al, 2006).
Usage and Biology
ADVANTAGES IN GENOME ENGINEERING APPLICATIONS: This part can be used to select positive integrants in the Synechocystis sp. PCC 6803 genome.
Improved Function
This part is an improvement to the documented part BBa_K300989. It has been optimized for use in the organism Synechocystis sp. PCC 6803, and also optimized to be compatible with iGEM assembly.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 135
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]