Difference between revisions of "Part:BBa K1507000:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The sequence may too long to cloning it from the E. coli K-12 strain.
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Containing promoter, ribosome binding site, FadR protein coding region and terminator. FadR protein is the translation product of this plasmid.
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Add another set of primer may be a good choice.
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===Source===
 
===Source===

Revision as of 02:46, 8 October 2014


PfadBA Repressor (J23119- B0034-FadR-B0015)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Containing promoter, ribosome binding site, FadR protein coding region and terminator. FadR protein is the translation product of this plasmid.

Source

E. coli K-12 strain

References