Difference between revisions of "Part:BBa K1465226:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Classical [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#BioBrick Suffix Insertion] of the sRNA:pfkA (<bbpart>BBa_K1465225</bbpart>) into the pSB1C3 backbone of the P<sub>tac</sub> promotor containing also the constitutive lacI repressor (<bbpart>BBa_K731500</bbpart>).
  
 
===Source===
 
===Source===

Revision as of 02:17, 8 October 2014


sRNA of phosphofructokinase A under the control of ptac promoter (ptac_sRNA:pfkA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1351
    Illegal XhoI site found at 1357
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Classical [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#BioBrick Suffix Insertion] of the sRNA:pfkA (BBa_K1465225) into the pSB1C3 backbone of the Ptac promotor containing also the constitutive lacI repressor (BBa_K731500).

Source

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References