Difference between revisions of "Part:BBa K1465225:Design"

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===Design Notes===
 
===Design Notes===
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*Amplification of the pSB1C3-Backbone (<bbpart>BBa_J04450</bbpart>) using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_sRNApfkA pSB1C3_pre_sRNApfkA] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_sRNApfkA pSB1C3_suf_sRNApfkA]:<br>
  
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'''pSB1C3_pre_sRNApfkA (43 bp)'''<br>
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5'-TGTGGCTAAAAGAATTAGTACTCTAGAAGCGGCCGCGAATTCC-3'<br>
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'''pSB1C3_suf_sRNApfkA (43 bp)'''<br>
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5'-TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTC-3'<br>
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*[http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson Gibson-Assembly] of the pSB1C3-Backbone and the gBlock containing the antisense RNA of the Phosphofructoskinase (''pfkA'') of ''Escherichia coli''.<br>
  
 
===Source===
 
===Source===

Revision as of 02:14, 8 October 2014


Knock-down of phosphofructokinase A using sRNA (sRNA:pfkA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 104
    Illegal XhoI site found at 110
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Amplification of the pSB1C3-Backbone (BBa_J04450) using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_sRNApfkA pSB1C3_pre_sRNApfkA] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_sRNApfkA pSB1C3_suf_sRNApfkA]:
pSB1C3_pre_sRNApfkA (43 bp)

5'-TGTGGCTAAAAGAATTAGTACTCTAGAAGCGGCCGCGAATTCC-3'

pSB1C3_suf_sRNApfkA (43 bp)

5'-TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTC-3'
  • [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson Gibson-Assembly] of the pSB1C3-Backbone and the gBlock containing the antisense RNA of the Phosphofructoskinase (pfkA) of Escherichia coli.

Source

Gensynthesis ([http://eu.idtdna.com/pages/products/genes/gblocks-gene-fragments gBlocks]) of the sRNA with the anti-sense RNA for the phosphofructokinase (pfkA) of Escherchia coli.

References