Difference between revisions of "Part:BBa K1465223:Design"

Revision as of 01:38, 8 October 2014

Functional carboxysome of Halothiobacillus neapolitanus with a GFP fusion under T7 control

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 216
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 374
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 882
Illegal AgeI site found at 1833
Illegal AgeI site found at 2514
Illegal AgeI site found at 3417
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 4566
Illegal SapI site found at 274

Classical [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#BioBrick Suffix Insertion] of the shell-associated proteins from Halothiobacillus neapolitanus (BBa_K1465204) into the pSB1C3 backbone of the T7 promotor (BBa_I719005).

Classical [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#BioBrick Prefix Insertion] of the shell-associated protein (BBa_K1465204) under the control of the T7 promotor (BBa_I719005) into the pSB1C3 backbone of the shell-proteins of the carboxysome from Halothiobacillus neapolitanus fused with GFP (BBa_K1465222).

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 ===Design Notes===
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+* Classical [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#BioBrick Suffix Insertion] of the shell-associated proteins from ''Halothiobacillus neapolitanus'' (<bbpart>BBa_K1465204</bbpart>) into the pSB1C3 backbone of the T7 promotor (<bbpart>BBa_I719005</bbpart>).
+* Classical [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#BioBrick Prefix Insertion] of the shell-associated protein (<bbpart>BBa_K1465204</bbpart>) under the control of the T7 promotor (<bbpart>BBa_I719005</bbpart>) into the pSB1C3 backbone of the shell-proteins of the carboxysome from ''Halothiobacillus neapolitanus'' fused with GFP (<bbpart>BBa_K1465222</bbpart>).
 ===Source===