Difference between revisions of "Part:BBa K1362415"
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<partinfo>BBa_K1362415 short</partinfo> | <partinfo>BBa_K1362415 short</partinfo> | ||
| − | This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's [[ | + | This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's [[2014.igem.org/Team:Heidelberg | wiki page]] as well as in RFC[???]. Specifically, this part contains the overhang B used to insert a protein in front of an N-Intein. It lies within the four bases formed by the cystein and the first base of the subsequent Leucine/Isoleucine or similar of the N-terminal splicing site. |
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Revision as of 13:02, 7 October 2014
N-terminal splicing overhang TGCT=Cys+1/3 Leu|Ile RFC[105] overhang NN
This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's wiki page as well as in RFC[???]. Specifically, this part contains the overhang B used to insert a protein in front of an N-Intein. It lies within the four bases formed by the cystein and the first base of the subsequent Leucine/Isoleucine or similar of the N-terminal splicing site.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]